Journal: Arthritis Research & Therapy

Article Title: Omentin-1 reduced synovial M1 macrophages through SIRT6 signaling pathway and alleviated knee osteoarthritis response in mice

doi: 10.1186/s13075-025-03680-y

Figure Lengend Snippet: Omentin-1 alleviated pain in ACLT induced SIRT6 −/− OA mice. A Timeline of animal experiments. We performed ACLT surgery to construct wild-type and SIRT6 −/− osteoarthritis (OA) mouse model, and injected 50µL Omentin-1 (300ng/joint/mouse) into the joint cavity of OA mice once a week for 5 consecutive weeks. Behavioral assessment was also performed through pain detection (PWTL) and gait analysis. Knee joint tissue was collected from mice at week 6, with a portion fixed in 4% paraformaldehyde for pathological staining and the remaining portion stored at −80℃ for Western blot analysis. The experimental groups were divided into three groups: Sham group, ACLT group, and Omentin-1 group, with 8 mice in each group. B Pain in OA mice was assessed before surgery and at 1, 2, 3, 4, 5, and 6 weeks after ACLT surgery. (C-E) At the 6th week after surgery, we conducted gait analysis and recorded the swing time, stride length, and paw area of the right hind limb. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: 24 h after ACLT surgery, two types of mice were injected with Omentin-1 (300ng/50μL, HY-P701099, MedChemExpress) [ ] twice a week into the joint cavity for six consecutive weeks.

Techniques: Construct, Injection, Staining, Western Blot